Green Synthesis and Characterization of Copper Nanoparticles using Madhunashini leaf extract and evaluation of its antibacterial property
Author(s):
Sweety Singh
Keywords:
Copper nanoparticles, Gymnema sylvestre, E. coli MTCC 40 and B. subtilis MTCC 2616, agar diffusion method
Abstract
Development of an environment friendly process for synthesis of NPs is significant step in the field of Nanotechnology. Among all the metallic nanoparticles, copper nanoparticles have drawn attention due to its unique physical, chemical and biological properties. In the present study, biosynthesis of copper nanoparticles was done using leaf extract of Gymnema sylvestre. The leaf extract of Gymnema sylvestre resulted in successful reduction of copper sulphate to Cu NPs as confirmed by UV-Visible Spectrophotometer by the presence of absorption peak at 651 nm. The average particle size of synthesized Cu NPs was found to be 111.9 nm by PSA. HRTEM images of Cu NPs showed the irregular ball like structures and their size was measured in the ranges from 10-24 nm. The antibacterial activity of the Cu NPs was evaluated against E. coli MTCC 40 and B. subtilis MTCC 2616 by agar well diffusion method. These results showed that Cu NPs synthesized from leaf extract of Gymnema sylvestre exhibited good antibacterial activity against gram +ve than gram –ve bacteria. These results confirmed that this method can be used for rapid and ecofriendly biosynthesis of stable Cu NPs.
Article Details
Unique Paper ID: 145278

Publication Volume & Issue: Volume 4, Issue 8

Page(s): 307 - 312
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