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@article{203190,
        author = {Vaishnavi S Marathe and Sandhya S Ahire and SUJEETKUMAR I AHIRE},
        title = {ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF CURCUMIN USING RP-HPLC TECHNIQUES},
        journal = {International Journal of Innovative Research in Technology},
        year = {2026},
        volume = {12},
        number = {12},
        pages = {12128-12134},
        issn = {2349-6002},
        url = {https://ijirt.org/article?manuscript=203190},
        abstract = {High Performance Liquid Chromatography (HPLC) is one of the most widely used analytical techniques. More than 85% of pharmaceuticals are analysed by HPLC.It has become mandatory to perform stability studies of a new drug moiety before filing in registration dossier. The stability studies include long term studies (12 months) and accelerated stability studies (25°C/60% RH or 40°C/75% RH, 6 months). But intermediate studies (6 months) can be performed at conditions milder than that used in accelerated studies. HPLC is essentially is a form of column chromatography in which the stationary phase consists of small particles (3-50µm) packing contain in column with small bore (2-5mm), one end of which is attached to a source of pressurized liquid eluent (mobile phase). Linearity range was 20-120µg/mL. Five replicate sample from the same batch were analysed by proposed method has been shown in the result within acceptance limit indicating reproducibility of the method. The accuracy of the method was ascertained on the basis of recovery study performed by the standard addition method. Stress degradation study was carried out on all the three developed methods as per ICH Q1A (R2) guidelines The mobile phase of HPLC ACN: aqueous buffer pH adjusted to 3.0 with phosphoric acid was selected},
        keywords = {HPLC, stability studies, ICH Q1A (R2), CURCUMIN, Stress degradation},
        month = {May},
        }