Impact of various temperature conditions and culture media on the development and sporulation of A. brassicae responsible for Alternaria blight in mustard

  • Unique Paper ID: 179944
  • Volume: 11
  • Issue: 12
  • PageNo: 8441-8445
  • Abstract:
  • Rapeseed-mustard (Brassica juncea L.) ranks as the second most significant oilseed commodity in India. Alternaria blight (A. brassicae) is a highly destructive fungal pathogen that leads to substantial yield losses in this crop. The current study was conducted to examine the impact of different temperature conditions and culture media on the mycelial growth and sporulation of A. brassicae. The results clearly indicate that all the temperature conditions and culture media tested exhibited variations in colony diameter and other cultural characteristics. A temperature of 25°C notably promoted mycelial growth (85.00 mm) and demonstrated excellent sporulation of A. brassicae when compared to 15 and 35°C. Regarding culture media, Potato Dextrose Agar proved to be significantly more effective than the other media, achieving maximum mycelial growth (82.00 mm) along with excellent sporulation. Conversely, Oat Meal Agar resulted in the least mycelial growth (45.00 mm) with only fair sporulation. Additionally, variations in cultural characteristics such as colony color, growth, appearance, and shape were also noted across the different culture media.

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