Evaluation of Micronuclei in Buccal cavity and Blood cells as a biomarker tool for Oral Cancer screening

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Priyanshu Singh; Ankur Chhari; Alibha Rawat; Sameena Akhter; Shivani Deshmukh; Shivani Tomar; Shweta Azad; Rachna Jain

Evaluation of Micronuclei in Buccal cavity and Blood cells as a biomarker tool for Oral Cancer screening

Authors: Priyanshu Singh, Ankur Chhari, Alibha Rawat, Sameena Akhter, Shivani Deshmukh, Shivani Tomar, Shweta Azad, Rachna Jain

Unique Paper ID: 182733
Volume: 12
Issue: 2
PageNo: 3075-3082

Keywords: Oral cancer screening Buccal mucosa cells Peripheral blood lymphocytes Cytogenetic biomarkers Oral squamous cell carcinoma (OSCC) Cytokinesis-block micronucleus (CBMN) assay.

Abstract:
The aim of this study was to describe the role of Micronucleus Frequency as a biomarker tool for Oral cancer screening & DNA damage compare with healthy individuals and oral cancer patients, through Buccal cells by BCMN assay and peripheral blood culture by (in vitro) CBMN assay. The frequency of MN has been shown to be greatly enhanced in patients with oral precancerous lesions and cancer when compared to healthy individuals. Peripheral blood lymphocytes have also been used to evaluate systemic genotoxic effects, for which higher MN frequencies have been found among oral cancer patients. Selection of patients/ volunteers, Oral swab and peripheral blood was collected from patients/ volunteers, Blood Culture, Harvesting of Micronucleus, Washing of Cells, Air Drop Method, Preparation of Slide, Giemsa staining, Microscopic observation of micronucleus morphology which shows genotoxic effects and DNA damage . A helpful biomarker for evaluating the effects of chromosome damaging substances and a trustworthy technique for assessing chromosomal damage in peripheral blood lymphocytes by cytokinesis-block micronucleus (CBMN) assay. According to this study, BCMN and CBMN with MN had a strong positive correlation, and the frequency of MN was linked to age and cancer stage. A notable rise in micronucleus formation in cancer patients suggestive of cytotoxicity or DNA damage. According to the study, oral cancer exhibits genotoxicity that is noticeably higher than that of healthy individuals. Buccal and blood samples can be used to predict the frequency of micronuclei, enabling an early assessment of genotoxicity prior to the emergence of clinical symptoms. This could be helpful in screening of the individual who are at high risk of cancer and early detection of those who have family history of cancer as high-risk individuals, such as smokers, tobacco chewers and alcohol users, according to the study, cytogenetic damage and the development of cancer are related.

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Cite This Article

ISSN: 2349-6002
Volume: 12
Issue: 2
PageNo: 3075-3082

Evaluation of Micronuclei in Buccal cavity and Blood cells as a biomarker tool for Oral Cancer screening

Available:https://ijirt.org/article?manuscript=182733

14 Jul, 2025

Genotoxicity estimation by calculating Mitotic index with Chromosomal aberrations induced by aqua-alcoholic extract of Annona muricata leave

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8.01 (Year 2024)

UGC Approved
Journal no 47859

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