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@article{185674,
        author = {EGLIN JUNO B K and Dr. A L Hema Latha},
        title = {Isolation, Media Optimization, and Industrial Application of Cellulase-Producing Bacteria from Coir Industry Waste soil},
        journal = {International Journal of Innovative Research in Technology},
        year = {2025},
        volume = {12},
        number = {5},
        pages = {2359-2367},
        issn = {2349-6002},
        url = {https://ijirt.org/article?manuscript=185674},
        abstract = {Cellulases are crucial biocatalysts for the sustainable conversion of lignocellulosic biomass into fermentable sugars, enabling biofuel, paper, and textile industries to reduce their reliance on chemical processes. This study reports the isolation, optimization, and industrial application of cellulase-producing bacterial strains, Bacillus sp. (S3) and Bacillus pacificus (S4), isolated from coir industry waste soils in Kanyakumari District, Tamil Nadu, India. The isolates were identified through morphological, biochemical, and molecular analyses, including 16S rRNA sequencing. Optimization experiments investigated the influence of pH, temperature, incubation time, carbon and nitrogen sources, and metal ions on cellulase production. The optimized conditions for Bacillus sp. (S3) were found at pH 6, 30 °C, and 36 h incubation, while Bacillus pacificus (S4) showed optimum production at pH 6, 30 °C, and 48 h incubation. Application studies demonstrated two major industrial utilities: (i) bioethanol production from coir pith hydrolysate, yielding 22.8 g/L reducing sugars and 10.1 g/L ethanol (S3), and (ii) textile biopolishing of cotton fabrics, enhancing smoothness and brightness by 38% and 12%, respectively. The findings highlight the dual industrial potential of coir-derived Bacillus strains for cost-effective enzyme production and sustainable bioprocess applications.},
        keywords = {Cellulase, Bacillus sp., Bacillus pacificus, Coir waste, Bioethanol, Biopolishing, Enzyme optimization},
        month = {October},
        }