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@article{207607,
        author = {ANBARASAN S and Mrs.R.KANMANI M.PHARM and ANBARASU C and ANANDHI P},
        title = {Antioxidant Potential of Calotropis gigantea (L.) Leaf Extract: A FRAP and CUPRAC Based Evaluation},
        journal = {International Journal of Innovative Research in Technology},
        year = {2026},
        volume = {13},
        number = {3},
        pages = {1767-1771},
        issn = {2349-6002},
        url = {https://ijirt.org/article?manuscript=207607},
        abstract = {The present study was undertaken to evaluate the in-vitro antioxidant potential of the leaf extract of Calotropis gigantea (L.) Dryand. (Apocynaceae), a widely distributed medicinal shrub traditionally used to treat inflammation, skin disorders and wounds, using ferric- and cupric-ion reducing assays. Shade-dried, powdered leaves of C. gigantea were extracted successively by maceration, Soxhlet extraction and magnetic stirrer-assisted extraction using an ethanol-water solvent system. The reducing/antioxidant capacity of the extract was determined by the Ferric Reducing Antioxidant Power (FRAP) assay, measured at 593 nm against a ferrous sulphate standard, and the Cupric ion Reducing Antioxidant Capacity (CUPRAC) assay, measured at 450 nm against a quercetin dihydrate standard. The Soxhlet and magnetic stirrer extractions yielded 1.54% and 3.17% w/w extract, respectively. In the FRAP assay, sample absorbance ranged from 0.250 to 0.757, corresponding to a reducing capacity of 25.6-77.5 µg/ml ferrous sulphate equivalent (mean 53.54 ± 19.67 µg/ml; R² = 0.919). In the CUPRAC assay, absorbance ranged from 1.13 to 3.94, corresponding to 33.3-116.2 µg/ml quercetin equivalent (mean 62.00 ± 33.09 µg/ml; R² = 0.842). Antioxidant capacity increased proportionally with absorbance in both assays. The leaf extract of Calotropis gigantea exhibited appreciable ferric- and cupric-reducing antioxidant capacity, supporting its traditional medicinal use and its candidacy as a natural antioxidant source for nutraceutical and pharmaceutical applications, warranting further evaluation by complementary radical-scavenging and in-vivo assays.},
        keywords = {Calotropis gigantea, Antioxidant activity, FRAP assay, CUPRAC assay, Leaf extract, Phytochemical screening, Free radical scavenging},
        month = {August},
        }