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@article{186475,
        author = {Shweta Patil and Abhishek Das},
        title = {Insilico Construction of Plasmid Containing Antimicrobial Peptides for Food Pathogens Along with Anticancer Peptides},
        journal = {International Journal of Innovative Research in Technology},
        year = {},
        volume = {12},
        number = {no},
        pages = {214-227},
        issn = {2349-6002},
        url = {https://ijirt.org/article?manuscript=186475},
        abstract = {The aim of this research is to identify novel antimicrobial peptides (AMPs) in fish species using bioinformatics tools, aiming to discover candidates for anticancer therapeutics and pathogen interaction. AMPs are small, positively charged molecules crucial in innate immunity, capable of killing pathogens and modulating host defenses, with some showing promise as anticancer agents. This study focuses on constructing plasmids containing AMPs with dual functionalities: combating food pathogens and exhibiting anticancer properties. Proteomic data from UniProt was analyzed, focusing on AMPs from snakehead murrel. Prediction tools like AMP Scanner, iAMPpred, AntiBP3, and CAMPR3 identified potential AMP candidates. Antigenicity and allergenicity of selected AMPs were evaluated using tools such as SVMTrip, Vaxijen, and AlgPred. Structural modeling and docking studies with CXCR1 and E.coli O157:H7 highlighted the potential of these AMPs, with promising results suggesting effective binding. These findings indicate that these AMPs could be valuable in food preservation and cancer therapy. Further studies could explore the in vivo efficacy of these peptides. This research underscores the promise of AMPs in medicine and food preservation, addressing antibiotic resistance and exploring their potential applications in cancer therapy.},
        keywords = {Antimicrobial Peptides (AMPs), docking, plasmids, structural modeling},
        month = {},
        }