Eclipta prostrata L. belonging to the family Asteracea is one of the oldest underutilized herbs used in traditional medicine. The plant is rich in bioactive components such as steroids, glucosides, tannins and saponins. It possesses antioxidant, antibacterial, antifungal and anti-inflammatory properties. The present work reveals the comparison of microwave assisted extraction with solvent extraction of polyphenols from Eclipta prostrata. Extraction of polyphenols was performed by varying the factors such as solvent concentration, extraction time and microwave power/temperature to analyze the effect of polyphenols. It is observed that the process parameters greatly influence the polyphenol content of the Eclipta prostrata extract. The yield of polyphenol was found higher in the microwave assisted extract than the macerated extract. This could be due to the rupture of cell wall at microwave power by the phenomenon of ionic conduction and dipolar rotation.