Antigenotoxic and Antiangiogenic Activity of Alternanthera Sessilis, Alstonia Scholaris and Anogeissus Acuminata Leaf Extracts.
Author(s):
Dr. Rashmi Mohapatra
Keywords:
Antiangiogenesis, Chromosomal aberration, Human lymphocytes, Mitotic Index
Abstract
Bioactive properties of Alternanthera sessilis, Alstonia scholaris and Anogeissus acuminata are extensively studied for their biological activities and being used in the treatment and management of human diseases in the traditional medical system. However, cytotoxic and genotoxic activities are yet to be explored. The present study was conducted to evaluate the cytotoxic and genotoxic effect in peripheral blood lymphocytes in vitro. As reports on the anticancer activities of these plants are scanty and expression of angiogenic factors reflect the aggressiveness of tumor cells, this study was undertaken to assess the pro/anti-angiogenic activity of these plant extracts in chick embryo chorioallantoic membrane. The presence of aberrant metaphase chromosome and mitotic index were evaluated in peripheral blood lymphocyte in vitro for genotoxicity and cytotoxicity assay. Genotoxicity and cytotoxicity assessments were carried out at the dose levels of 75, 150 and 300 µg/ml while antiangiogenic activities were studied at 25, 50 and 100 µg/ml concentration. Experiments were performed in triplicates for each concentration. Genotoxicity and Cytotoxicity test resulted in the absence of toxicity at doses between 75 µg/ml and 300µg/ml. Leaf extracts of all the three plants showed antiangiogenic activity at the dose level tested and it was dose specific. The percentage of inhibition of neovascularization in A.sessilis extract were 26%,82% and 91% and in A.scholaris 91%,97%,98%, at the dose level of 25, 50 and 100 µg/ml where as in A.accuminata the percentage reduction are 65%,76%,88% from lower to higher concentration. The selected doses of the plant extract were not genotoxic and cytotoxic but they have a high potential antiangiogenic activity.
Article Details
Unique Paper ID: 152594

Publication Volume & Issue: Volume 8, Issue 3

Page(s): 895 - 902
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