MEMBRANE-SUPPORTED LIQUID-LIQUID-LIQUID MICRO EXTRACTION COMBINED WITH ANION-SELECTIVE EXHAUSTIVE INJECTION CAPILLARY ELECTROPHORESIS-ULTRAVIOLET DETECTION FOR SENSITIVE ANALYSIS OF PHYTOHORMONES
Author(s):
SELVI S R
Keywords:
Phytohormones, Membrane-supported liquid–liquid–liquid, Micro Extraction, Anion-selective Exhaustive Injection, Capillary Electrophoresis
Abstract
A novel method based on off-line membrane-supported liquid–liquid–liquid micro extraction (MS-LLLME) combined with on-column anion-selective exhaustive injection (ASEI) capillary electrophoresis–ultraviolet (CE–UV) detection was established for the analysis of seven phytohormones (abscisic acid (ABA), jasmine acid (JA), 2,4-dichlorophenoxyacetic acid (2,4-D), 1-naphthalene acetic acid (NAA), indole-3-acetic acid (IAA), salicylic acid (SA) and gibberellic acid (GA)). In MS-LLLME, the target phytohormones were extracted from the acid donor phase to the alkaline acceptor phase, and the acceptor solutions were directly analyzed by ASEI-CE–UV. Under the optimal experimental conditions, the analytical performance of the method was evaluated. The limits of detection (LODs) of ABA, JA, 2,4-D, NAA, IAA, SA and GA were determined to be 1.00, 2.21, 0.33, 0.17, 0.67, 0.05 and 16.5 ng/mL, respectively. The relative standard deviations (RSDs, n = 7) ranged from 4.7% to 12.9%, and the enrichment factors were in the range of 307 to 20,160. The proposed method was successfully applied for the determination of multiple phytohormones in banana, cabbage and cucumber extracts, and ABA, IAA and SA were detected in these samples. The recoveries for the spiked samples were in the range of 79.0 to 116.4%. The proposed method was demonstrated to be suitable for the simultaneous quantification of multiple phytohormones with high sensitivity and good sample cleanup ability
Article Details
Unique Paper ID: 146904

Publication Volume & Issue: Volume 5, Issue 2

Page(s): 407 - 413
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