Standardization of Regeneration Protocol Through Somatic Embryogenesis in Banana, cvs. Rasthali
Author(s):
Dr. Keshamma E, Prathibha. K. Y, Manjula. A. C., Mohandas. S
Keywords:
Banana, cv. Rasthali, Somatic embryogenesis, Regeneration protocol, Callus induction.
Abstract
The present study was designed with the main aim to standardization of regeneration protocol through somatic embryogenesis in banana, cvs. Rasthali (AAB). The male flower buds and suckers of banana cvs. Rasthali were collected periodically from Hesaraghatta and Nanjangud. The explants were cultured within 24 hours of collection. Experiments were conducted using Murashige and Skoog’s medium (MS) with different plant growth regulators and MS medium was found to be the best for establishment of various cultures because of high concentrations of mineral salts. Growth regulators viz., auxins, cytokinins, gibberellins and abscisic acid were used in tissue culture experiments. Various experiments were carried out in order to standardize the media composition and type of growth regulators to obtain highest frequency of plantlets through germination of somatic embryos. A statistical analysis of the response of the various explants was done using GraphPad Prism Software. P<0.05 was considered statistically significant. The results revealed that Banana cvs. Rasthali (AAB) are seriously under the threat of extinction due to its susceptibility to fusarium wilt. A regeneration protocol using somatic embryogenesis was developed in this study, using immature flower buds. This reliable regeneration method is a prerequisite for molecular manipulation to obtain disease resistance bananas. Immature flower buds cultured in-vitro resulted in embryogenic callus formation in this study in banana cvs. Rasthali (AAB). Secondary somatic embryo formation was commonly observed on the primary embryos of banana cv. Rasthali. In summary, banana cv. Rasthali the cvs. exhibited differential response to embryo development depending on their genotype. The study has shown immature flower buds were better choice of explants to induce embryogenic callus than shoot tip explants in banana cv. Rasthali. Nodular yellowish green callus was the most common non-embryogenic callus observed in this study.
Article Details
Unique Paper ID: 152411

Publication Volume & Issue: Volume 8, Issue 3

Page(s): 302 - 318
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