EFFECT OF HERBICIDES ON POLLEN GERMINATION AND POLLEN TUBE GROWTH IN VITRO
Author(s):
Mhaiskar MN
Keywords:
Herbicide, Pollen germination, Pollen tube, Sida acuta
Abstract
The pollen of weed Sida acuta Burm.f. were germinated in different concentrations of herbicides by adopting ‘Hinging drop’ technique. The effects of herbicides on pollen germinations and pollen tube growth were observed till the lethal dose was found. The pollen germination and pollen tube growth gradually decreased with the increase in concentration of herbicide. The lethal doses for pollen germination were observed at 50, 50 and 50 ppm of 2,4-D, stomp and glyphosate, respectively. At these concentrations the bursting observed. So there was no germination of pollen and no growth of pollen tube found. The percentage of pollen germination prior to lethal dose found at 40, 40 and 40 ppm was 43.75, 59.09 and 46.00 micron, respectively with pollen tube length counted was 62.50, 62.50 and 79.21 micron, respectively in 2,4-D, stomp and glyphosate, respectively against 84.32 % pollen germination with 287.50 micron pollen tube length in control. At the time of pollen germination and pollen tube formation, abnormalities like bursting an swelling were the common at all concentrations of herbicides. 2,4-D was more effective than stomp and glyphosate for inhibiting the pollen germination and pollen tube growth.
Article Details
Unique Paper ID: 152486

Publication Volume & Issue: Volume 8, Issue 3

Page(s): 570 - 574
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