High efficiency direct organogenesis of Bacopa monnieri - A highly valuable medicinal plant
Bacopa monnieri, direct organogenesis, leaf explants, phytochemical profile
Bacopa monnieri, popularly known as “Brahmi”, a member of the Scrophulariaceae family, is a small, creeping herb grows in damp, marshy areas. It is a reputed medicinal herb in Indian systems of medicine. It is also under cultivation as medicinal crop. Traditionally, it was used as a brain tonic to enhance memory development, learning, and concentration, and to provide relief to patients with anxiety or epileptic disorders. The demand of this plant in the medicinal world is very high as compared to its supply. In the present work efficient protocol was developed for large scale mass propagation of this valuable medicinal plant. In vitro regeneration by direct organogenesis was achieved using nodes, internodes and young leaves. Maximum response of 99.82 + 0.7 with a very high number of shoot bud (120 + 0.3 ) was induced from leaf explants on Murashige and Skoog (MS) medium supplemented with 0.5 mg L-1 BA (6-benzyladenine) and 0.1 mg L-1 IAA ( Indole-3-Acetic Acid). 100%of the regenerated shoots produced root on MS medium containing 0.1 mg L-1 IAA with maximum number of 14.5 + 0.4 observed after 2 weeks of subculture. The rooted shoots were hardened and were successfully established in soil with a survival rate of 95%. HPTLC (High-performance thin-layer chromatography) profile of the methanolic extracts of the in vitro raised plants and mother plants revealed almost identical result with no significant differences.
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Unique Paper ID: 152660

Publication Volume & Issue: Volume 8, Issue 4

Page(s): 1058 - 1664
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