Formulation and  evaluation of novel herbal combination for Anti-urolithiasis activity

Formulation and evaluation of novel herbal combination for Anti-urolithiasis activity
Author(s):
Pratiksha Nikam, Madan Pomaje
Keywords:
Urinary calculi, Calcium oxalate, Punarnava, Artificial urine, invitro assay.
Abstract
The presented study was being carried out to evaluate the invitro anti-urolithiasis activity of a novel combination including roots and seeds of Punarnava and Cotton seed. The in-vitro inhibition of commonly observed calcium-oxalate crystal in most of the urinary caliculi was been carried out by using a combination of extract by different assay procedures. Artificial urine was prepared by supersaturating with calcium oxide and urolithiasis was studied by Nucleation assay and Aggregation assay. Crystal inhibition in artificial urine was studied at different time intervals using root and seed extract at different concentrations 50, 100, 150, 200,250 mg/µl each respectively. The comparison study was carried out between the selected combination and marketed formulation Cystone (Control) .
Article Details
Unique Paper ID: 153828 Publication Volume & Issue: Volume 8, Issue 9 Page(s): 60 - 65
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