Extraction and Screening of Exopolysaccharide Producing Bacteria from Curd
Shrikant B. Bhosale, Amarsinh B. Varpe, Arjun Choudhari, Shubham Solanke, Mahesh Virmale, Deepak Garandwal, Pravin Pakhare, Vipin Hiremath
Exopolysaccharide (EPS), emulsification, FTIR, Biochemical and Morphological analysis.
Exopolysaccharide (EPS) are environment friendly natural polymers consist of sugar moieties and secreted by microorganisms extracellularly having high molecular weight polymers. EPS has diverse applications in the food industry. In the present work, the two bacteria producing higher EPS was screened among eight other bacterial isolates from curd. The samples were plated on MRS agar plates and colonies showing ropiness, sticky and mucoid consistency were selected. The isolates were further used for EPS production and the only bacterium producing higher amount EPS used for further work. The identification and characterization of this superior EPS producing bacterium were done based on biochemical and morphological analysis. The EPS production carried out in a modified EPS selection medium (mESM) for about 48 hrs in shaking conditions. The extraction was done with the two-volume of prechilled isopropanol. The extracted EPS was analyzed by using the FTIR technique for functional group detection. We also analyzed for its emulsification activity. The emulsification was carried out with different oil as well as chemical hydrocarbons including benzene. The results were compared with standard surfactant i.e. Triton X-100. The EPS showed good emulsification activity against different oils. Thus, indicated its possible use as an additive in the food industry.
Article Details
Unique Paper ID: 153959

Publication Volume & Issue: Volume 8, Issue 9

Page(s): 347 - 353
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