Synthesis and Characterization of Silver nanoparticles using Justicia adhatoda(leaf) and its Pharmacological Activity
Author(s):
SUGUNA.S, V.S.Sangeetha, V.Bhakkiyajothi, G.Saranya
Keywords:
Justicia adhatoda (leaf), Silver nanoparticles, Silver nitrate, UV, XRD, FTIR, Antibacterial activity, etc.
Abstract
Nanotechnology is an emerging field in Biotechnology as the Green technology works with nanotechnology to develop significant and practical protocols when manufacturing nanotechnology-enabled products. By modifying the production process, the risks that are associated with nano-products are minimized and the green products can be used for environmental applications. Biomolecules present in herbal extracts can be used to reduce metal ions to nanoparticles in a single-step green synthesis process. This biogenic reduction of metal ion to base metal is quite rapid and they readily conduct at room temperature, pressure and can easily scale up. Silver nanoparticles are reported to have magnetic, catalytic, optical and anti-inflammatory activity. In this study, Silver nanoparticles were successfully synthesized by reduction of Silver nitrate solution using aqueous solution of Justicia adhatoda (leaf) extract. The method was non-lethal, simple, eco-accommodating and relatively inexpensive. The resulting Silver nanoparticles were characterized by physical color changes, UV-Vis spectroscopy, and Fourier-transform infrared (FTIR) spectroscopy. The reaction mixture of silver nitrate and Justicia adhatoda (leaf) extract exhibited black color confirming the synthesis of silver nanoparticles. UV–Vis spectra showing an absorption band at 300 to 450 nm is the characteristic of the Silver nanoparticles. Also, in deed revealed the presence of nanoparticles. From the spectrum of the biosynthesized Silver nanoparticles at different magnifications from XRD and TEM showed that the average particle size of the nanoparticles is found to be 25 nm. FTIR spectra, the absorption band at 3887.9 cm−1 which is characteristic of the OH stretching of phenolic group. The absorption bands at 1173.8 and 1605 cm−1 correspond to carbonyl group present in the extract; synthesized Silver nanoparticles had significantly higher Anti- inflammatory activity by Albumen Denaturation method.
Article Details
Unique Paper ID: 155910

Publication Volume & Issue: Volume 9, Issue 2

Page(s): 730 - 737
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