Possible interaction of water- soluble vitamins with nitric oxide and its significance - Evidence from Reversed Phase-High Pressure Liquid Chromatography (RP-HPLC)
Rita Bhatla, Mansi Gogna, Renu Aggarwal, Vishnu Bhat
Water-soluble vitamins, Nitric Oxide, RP-HPLC, ROS scavenging
Water soluble vitamins (B series and C) are physiologically significant biomolecules since one of the major roles for some of them is to scavenge metabolically harmful reactive oxygen species (ROS) produced in living beings. Nitric oxide (NO), a naturally produced gaseous and reactive biomolecule, also serves as an antioxidant in cell metabolism depending majorly on its endogenous concentration. In view of these apparently similar biochemical roles of water-soluble vitamins and NO, authors have attempted to investigate possible interaction between them. In this regard, evidence has been generated from RP-HPLC coupled with UV detector. Interestingly, all water-soluble vitamins, except vitamin B2 (riboflavin) and B5 (calcium D-pantothenate), exhibited noteworthy changes in their respective retention times and/ or significant changes in the intensity of peak absorbance upon interaction with equimolar concentration of NO donor (Diethylenetriamine NONOate; DETA). Vitamin B7 (D-Biotin) and B6 (pyridoxine hydrochloride) showed a very significant increase in their respective peak absorbance (112.5 and 11.33 fold, respectively) at 247 nm. These observations provide new evidence for possible interaction of NO with some of the water-soluble vitamins and offer novel mechanisms of action of water-soluble vitamins as ROS scavengers in biological systems.
Article Details
Unique Paper ID: 156401

Publication Volume & Issue: Volume 9, Issue 3

Page(s): 547 - 557
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