Development and validation of UV Spectrophotometric method for estimation of β-carotene in Cissus quadrangularis stem extract
Author(s):
Maske Rajratna Ashokrao, Dr. J.Y. Nehete, Aishwarya D. Ghuge
Keywords:
UV- visible spectrophotometry, β-carotene, Cissus quadrangularis, validation.
Abstract
A simple, precise and cost effective UV- visible spectrophotometric method for the estimation of β-carotene in Cissus quadrangularis stem extracts was developed and validated according to the ICH Q2 (R1) guideline. β-carotene is a carotenoid and a precursor of vitamin A, which is isolated from the stems of Cissus quadrangularis plant. Spiked β-carotene solution was scanned over UV-visible range for its wavelength of maximum absorbance. Various calibration standards of β-carotene were prepared. Calibration curve of concentration vs. absorbance was plotted. Various analytical method validation parameters viz. linearity, accuracy, precision, robustness, ruggedness, limit of detection and limit of quantitation were calculated. The maximum wavelength of β-carotene was found to be 459nm. The correlation coefficient over the concentration range of 5-25 μg/ml was found to be 0.9983. Developed UV method was found to be precise for the intra-day and inter-day study and shows percent relative standard deviation in the range of 0.71 & 1.04 to 0.62 to 0.92 respectively. The total percent recovery of β-carotene was found to be 98.80 to 100.88%. A simple, precise and cost effective UV- visible spectrophotometric method for the estimation of β-carotene in standardized extract of stems of Cissus quadrangularis was developed. The present validated UV- visible method can be efficiently used for the estimation of β-carotene in extracts of Cissus quadrangularis stems.
Article Details
Unique Paper ID: 156410

Publication Volume & Issue: Volume 9, Issue 3

Page(s): 659 - 665
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