Analytical Method Development And Validation Of L-Arginine Effervescent Powder

  • Unique Paper ID: 158585
  • Volume: 9
  • Issue: 10
  • PageNo: 200-205
  • Abstract:
  • A simple, selective, rapid, precise and economical reverse phase HPLC method has been developed for the L-Arginine effervescent powder. The method was carried out on a Inertsil C8 (250 x 4.6 mm x 5 μm)) column with a mobile phase consisting of Solution A: Acetonitrile (920:80) where solution A contain 500 mg of octane sulphonic acid in 1000 ml of phosphate buffer (pH 3.5) at a flow rate of 1.0 ml/min. Detection was carried out at 215 nm. The retention time of L-Arginine was found to be 7.5 minutes. The developed method was validated in terms of system suitability, linearity, accuracy& %recovery, precision, robustness, specificity, intermediate precision. The proposed method can be used for the estimation of L-Arginine. The proposed method is optimized and validated as per ICH guidelines.

Copyright & License

Copyright © 2025 Authors retain the copyright of this article. This article is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

BibTeX

@article{158585,
        author = {Ashpak Inamdar  and Dr. Ameya Mulay and Mahendra Godase and Mukta Rankhamb and Sonal Gawali},
        title = {Analytical Method Development And Validation Of L-Arginine Effervescent Powder},
        journal = {International Journal of Innovative Research in Technology},
        year = {},
        volume = {9},
        number = {10},
        pages = {200-205},
        issn = {2349-6002},
        url = {https://ijirt.org/article?manuscript=158585},
        abstract = {A simple, selective, rapid, precise and economical reverse phase HPLC method has been developed for the L-Arginine effervescent powder. The method was carried out on a
Inertsil C8 (250 x 4.6 mm x 5 μm)) column with a mobile phase consisting of Solution A: Acetonitrile (920:80) where solution A contain 500 mg of octane sulphonic acid in 1000 ml of phosphate buffer (pH 3.5)
at a flow rate of 1.0 ml/min. Detection was carried out at 215 nm. The retention time of L-Arginine was found to be 7.5 minutes. The developed method was validated in terms of system suitability, linearity, accuracy& %recovery, precision, robustness, specificity, intermediate precision.
The proposed method can be used for the estimation of L-Arginine. 
The proposed method is optimized and validated as per ICH guidelines.},
        keywords = {Analytical method development, RP-HPLC, L-Arginine},
        month = {},
        }

Cite This Article

  • ISSN: 2349-6002
  • Volume: 9
  • Issue: 10
  • PageNo: 200-205

Analytical Method Development And Validation Of L-Arginine Effervescent Powder

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