Analytical Method Development And Validation Of L-Arginine Effervescent Powder
Author(s):
Ashpak Inamdar , Dr. Ameya Mulay, Mahendra Godase, Mukta Rankhamb, Sonal Gawali
Keywords:
Analytical method development, RP-HPLC, L-Arginine
Abstract
A simple, selective, rapid, precise and economical reverse phase HPLC method has been developed for the L-Arginine effervescent powder. The method was carried out on a Inertsil C8 (250 x 4.6 mm x 5 μm)) column with a mobile phase consisting of Solution A: Acetonitrile (920:80) where solution A contain 500 mg of octane sulphonic acid in 1000 ml of phosphate buffer (pH 3.5) at a flow rate of 1.0 ml/min. Detection was carried out at 215 nm. The retention time of L-Arginine was found to be 7.5 minutes. The developed method was validated in terms of system suitability, linearity, accuracy& %recovery, precision, robustness, specificity, intermediate precision. The proposed method can be used for the estimation of L-Arginine. The proposed method is optimized and validated as per ICH guidelines.
Article Details
Unique Paper ID: 158585

Publication Volume & Issue: Volume 9, Issue 10

Page(s): 200 - 205
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