Progression of loop-mediated isothermal amplification (LAMP) assay for detection of Sitophilus oryzae in food samples
Author(s):
Trupti N. Barbuddhe
Keywords:
Sitophilus oryzae, LAMP, stored product insect, food matrices
Abstract
Post-harvest crop losses, due to insects and microbes, accrue significant economic waste in both developed and developing countries. The rice weevil (Sitophilus oryzae) is a stored product pest which attacks several crops, including wheat, rice, and maize. S. oryzae is able to develop on a wide range of cereals and also on processed cereal products such as pasta. A few strains of S. oryzae have been found which can develop on grain legumes peas, lentils and green or black grams are the pulses most often attacked by these strains. The infestation of stored product insects leads to the qualitative and quantitative deterioration of the commodity as they feed on them. The stored product may contain either adults, larvae, eggs or dead insect parts. Hence the aim of this work is to develop a isothermal amplification techniques to detect the eggs, larvae, adult insect and their remnants in different food matrices using specific primers. Primers were designed for ‘internal transcribed spacer 2’ genes of Sitophilus and LAMP conditions were optimized for the detection of Sitophilus oryzae. The Sitophilus oryzae contaminated food matrices were checked for matrix interference in LAMP analysis.
Article Details
Unique Paper ID: 164652

Publication Volume & Issue: Volume 10, Issue 12

Page(s): 2126 - 2131
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