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Isolation, Screening and Molecular characterization of Lipase producing Bacteria and its application in detergents

  • Unique Paper ID: 165192
  • Volume: 11
  • Issue: 1
  • PageNo: 712-719
  • Abstract:
  • This study aims to investigate the commercial potential of lipases produced by bacteria. Four isolates were obtained from the lake water. A unique lipolytic medium with 1% (w/v) mustard oil was used to cultivate the isolated lipolytic bacteria. Egg yolk was used in the tests to measure primary lipase activity. The maximum lipolytic activity enzyme extracts from Isolate-2 and Isolate-4 were used for further use. The maximum displacement area was ascertained by separating the displaced oils (mustard oil and vegetable oil) before analyzing the oil displacement tests. The Isolate-2 was identified as Pseudomonas aeruginosa through 16S rRNA gene sequence analysis. The efficiency of employing crude enzyme extracts to remove oil stains from cotton fabric pieces was assessed. The lipase enzyme has oil stain removal properties and has proven effective as a detergent additive.
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Cite This Article

  • ISSN: 2349-6002
  • Volume: 11
  • Issue: 1
  • PageNo: 712-719

Isolation, Screening and Molecular characterization of Lipase producing Bacteria and its application in detergents

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