simultaneous estimation of Ampicillin, Amoxicillin, Potassium Clavulanate & Penicillin v Potassium by RP_HPLC method in Pharmaceutical waste
Author(s):
Mohammadi Amjad, Dr Swetha Addanki, Dr Jabeen Farhana, Dr Swarna Bharathi
Keywords:
Pharmaceutical waste, RP-HPLC, PDA, Method validation, Octa decyl silyl carbon
Abstract
The assay of Clavulanate potassium, Ampicillin, Amoxicillin and Penicillin V Potassium was performed with pharmaceutical waste and the % assay was determined from pharmaceutical waste. The chromatographic Method conditions were done in WATERS HPLC, software: Empower, 2695 separation module, PDA as a analyzer and LABINDIA and UV 3000 UV Spectrophotometer,Inertsil ODSC18 with flow rate 1ml/min, at ambient column temperature, injection volume 20 μl, detection wavelength 295nm and prepared mobile phase of Phosphate buffer pH 3: Acetonitrile (30:70). Assay Result for Clavulanate potassium, Ampicillin, Amoxicillin and Penicillin V Potassium was performed with pharmaceutical waste and the % assay was found to be 16.21, 15.54, 16.80 and 14.84 from pharmaceutical waste. Stress degradation studies were performed on the Clavulanate potassium, Ampicillin, Amoxicillin and Penicillin V Potassium using the proposed method. And all the samples degraded within the limits. Concluding sensitive assay method is validated for drugs such as Amoxicillin, Ampicillin, Penicillin V Potassium and Clavulanic Acid. Using a simple buffer and Isocratic programming, an accurate simultaneous determination of these drugs has been made possible at very low level is demonstrated. Author believes that this method is very useful in testing of public sewer waste water and is less expensive.
Article Details
Unique Paper ID: 166184

Publication Volume & Issue: Volume 11, Issue 2

Page(s): 321 - 330
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