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Biotechnological aspect of LAMP in Agriculture

  • Unique Paper ID: 166610
  • Volume: 11
  • Issue: 2
  • PageNo: 1343-1345
  • Abstract:
  • LAMP has gained a significant interest in research across a diverse field of Agriculture. This technology is considered an established method for amplifying nucleic acid and also for diagnosis of field diseases. Due to its cost effectiveness when compared to PCR, LAMP method is considered to be fast and precise and large amount of DNA is produced. This technology was invented in 1998 by Eiken Chemical Company Tokyo. LAMP amplification is carried out at constant temperature and there is no need for the use of thermal cycler. Target DNA sequence is amplified at 60-65°C.Two or three set of primers along with polymerase like Bst Klenow fragment with high strand displacement activity in addition to replication activity is used. Amplification in LAMP can be detected using photometry and by measuring turbidity which is caused by Magnesium pyrophosphate precipitate in solution it is formed as a result of byproduct of amplification that allows easy visualization by naked eyes and also by simple photometric detection. Certain dyes recommended for LAMP that induce a visible colour change are SYBR green which allows visible colour change with naked this occurs as dye molecule intercalates DNA. Other methods for visualization by LAMP is by hybridization with complementary gold nanoparticle bound to SS DNA.
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  • ISSN: 2349-6002
  • Volume: 11
  • Issue: 2
  • PageNo: 1343-1345

Biotechnological aspect of LAMP in Agriculture

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