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@article{156091,
        author = {Darshana Dhokane and Dipali Girase and Ashwini Kadbhane},
        title = {Recent Advancement in Genetic engineering using DNA manipulating enzymes},
        journal = {International Journal of Innovative Research in Technology},
        year = {},
        volume = {9},
        number = {2},
        pages = {819-822},
        issn = {2349-6002},
        url = {https://ijirt.org/article?manuscript=156091},
        abstract = {The development of molecular genetics as well as genetic manipulation are leading to innovative changes in the field of life science. Genetic engineering is an accurate technique of gene manipulation at the molecular level. Several enzymes play an important role in genetic engineering like restriction endonuclease, ligase, Alkaline phosphatase as well as DNA polymerase. The importance of genetic engineering in medical science consists of the formation of vaccines, growth hormones including proteins along with the treatment of various diseases. Genetic engineering has several applications in agriculture, research, medicine as well as industrial biotechnology. CRISPR/Cas9 is the new genome editing technique with excellent advantages. The fast advancement in CRISPR/Cas9 system greatly revolved around genetic engineering, particularly modifications of the Cas9 as well as sgRNA to attain proper editing.},
        keywords = {Genetic Engineering, CRISPR-Cas9, DNA Manipulation, Genome},
        month = {},
        }