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@article{170512,
        author = {Mr. Abhishek Raosaheb Ghule and Mr. Kuldeep Sukhdeo Kanadje and Dr. D. K. Vir and Mr. Vaibhav Manikrao Hiwale and Miss. Pooja Dadarao Gayake and Mr. Yash Kishor Pendharkar},
        title = {Systemic Review on CRISPR-CAS9 Technology: Unleashing the Power of Precision Genetic Editing},
        journal = {International Journal of Innovative Research in Technology},
        year = {2024},
        volume = {11},
        number = {7},
        pages = {325-332},
        issn = {2349-6002},
        url = {https://ijirt.org/article?manuscript=170512},
        abstract = {CRISPR-Cas9 technology has emerged as a transformative tool in genetic editing, offering unparalleled precision and efficiency. Originally discovered as a bacterial defense mechanism against viral infections, this system enables precise targeting and modification of specific DNA sequences. This article aims to examine the potential applications of CRISPR-Cas9 in addressing significant challenges in healthcare, agriculture, and environmental science. The technology relies on two critical components: the Cas9 enzyme, which acts as molecular scissors, and a guide RNA (gRNA) that directs the enzyme to the desired DNA site. By designing tailored gRNAs, scientists can achieve highly accurate gene editing. This method has shown significant promise in correcting genetic disorders, treating cancers, and addressing infectious diseases, as demonstrated by ongoing clinical trials. In agriculture, CRISPR-Cas9 has been utilized to develop crops with enhanced resistance to pests, diseases, and environmental stresses, contributing to improved food security and sustainability. While the results highlight CRISPR-Cas9's potential, challenges such as unintended off-target effects and the ethical concerns of germline editing, which affects future generations, necessitate careful evaluation. Establishing robust ethical guidelines and fostering public discussions are essential to ensure its responsible application. In summary, CRISPR-Cas9 is a groundbreaking innovation in genetic editing, offering transformative solutions for global health and agricultural challenges. However, addressing its safety and ethical implications is crucial to realizing its full potential. Continued research and dialogue will play a pivotal role in shaping its future use responsibly.},
        keywords = {CRISPR–Cas9; Gene Editing; Molecular Genetics; Prime Editing.},
        month = {December},
        }