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@article{172269,
        author = {Dr. Mitesh Bhatt and Dr. Akash Shah and Dr. Sandip Shah and Dr. Bhavini Shah and Mrs. Ankita Murnal},
        title = {Simultaneous separation and quantitation of five Biogenic amines in urine by liquid chromatography–ionspray tandem mass spectrometry with multiple-reaction},
        journal = {International Journal of Innovative Research in Technology},
        year = {2025},
        volume = {11},
        number = {8},
        pages = {2653-2658},
        issn = {2349-6002},
        url = {https://ijirt.org/article?manuscript=172269},
        abstract = {A reliable, selective and sensitive liquid chromatography tandem mass spectrometry method was developed and validated for the quantification of Biogenic amines in urine. Chromatographic separation was performed on a Shim pack GIST C18, 4.6*75mm, 3µm using lc gradient methanol: 0.1% formic acid solution as the mobile phase at a flow rate of 0.300 mL/min. We describe sample processing and analysis methods for simultaneous quantification of multiple biogenic amines, in urine using LC–MS/MS. A simple extraction procedure technique compatible with each functional group for measuring VMA, HIAA, HVA, Hydroxyproline and 3-Methoxytyramine, with high sensitivity and specificity is also described. The positive and negative ionization mode with multiple reaction monitoring was used for detection. The sensitivity was good with no carry-over detected and the lower limit of quantification range. The method has been successfully verified using authentic case samples that had previously been quantified using different methods. The assay is suitable for clinical utilization and management of patients on these medications.},
        keywords = {Biogenic amines; Therapeutic drug monitoring; UPLC-MS/MS; human urine},
        month = {January},
        }