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@article{178943,
        author = {Indrani Chandra},
        title = {Study of indirect organogenesis of Coccinia grandis (L.)Voigt by using Fluorescence and Scanning Electron Microscope},
        journal = {International Journal of Innovative Research in Technology},
        year = {2025},
        volume = {11},
        number = {12},
        pages = {6388-6393},
        issn = {2349-6002},
        url = {https://ijirt.org/article?manuscript=178943},
        abstract = {Coccinia grandis(L.)Voigt is a well-known dioecious plant of Cucurbitaceae.The plant is used as a raw vegetable as well as in the treatment of many diseases like skin disease, bronchitis, diabetes, etc. Different explants such as internode, node, leaf and shoot apex were used for callus induction. In vitro regeneration of this plant was obtained by transferring the explants into Murashige and Skoog’s (MS) media supplemented with 3.0% sucrose, 0.8% agar. The PGRs used 2,4D, NAA, BAP and Kin at different combinations and concentrations. Maximum root regeneration was obtained from nodal explants derived callus i.e.,9.2 ± 0.27 in 2.0 mg/l NAA. Maximum shoot bud regeneration from callus i.e, 12.3±0.31 was obtained in 0.1mg/l NAA and 1.0 mg/l BAP. Regeneration of shoot bud and root were confirmed by scanning electron microscope. Histological study of regenerated shoot bud with distinct shoot apex was observed through Fluorescence Microscope.},
        keywords = {MS; NAA; BAP; KIN; Histology; Dioecious},
        month = {May},
        }