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@article{182578,
author = {Dr. Siddharth N. Mendhe and Dr. Anil M. Garode},
title = {Isolation and Identification of Actinobacteria (Kocuria sp., Conexibacter sp., Brevibacterium sp. and Rubrobacter sp.) from Lonar lake District Buldhana, Maharashtra.},
journal = {International Journal of Innovative Research in Technology},
year = {2025},
volume = {12},
number = {2},
pages = {2658-2667},
issn = {2349-6002},
url = {https://ijirt.org/article?manuscript=182578},
abstract = {During the study out of 60 water samples 04 actinomycete isolates were obtained which were labelled from ACM1 to ACM 4. All samples are collected from the five different sample collection sites from the Lonar lake area were selected for the collection of water samples. Such as Dhar water spot, Ramgaya Spot, Kamalaji Devi Temple spot, Ambarkhana Mahadev Temple spot, Mungala Mahadev Temple spot. The present study was performed by collection and examination of Lonar Lake water samples during three different seasons. Samples were collected in sterile bottles and later transferred to P. G. Department of Microbiology, Shri Shivaji Science and Arts College, Chikhli, Dist. Buldhana for further study. It includes Physicochemical Analysis of water, Salinity (By using Chloride content), Isolation of diversity of Actinomycetes from water and sediment samples of Lonar Crater, Characterization and identification of Actinobacteria, Identification of actinobacteria on the basis of 16s RNA, Extraction of genomic DNA Phylogenic analysis of actinomycetes. The genomic DNA of 4 Actinomycetes isolates was extracted by using a commercially available kit according to the manufacturer’s guide. We isolates four actinomycete (Kocuria sp., Conexibacter sp., Brevibacterium sp. and Rubrobacter sp.) and complete his further study. We have received accession numbers for all the sequences. The isolated actinomycetes species were ACM 01 Kocuria rhizophila (ON248525), ACM 02 Conexibacter woesei (ON377276), ACM03 Brevibacterium spp., ACM 04 Rubrobacter xylanophilus (ON387757).},
keywords = {Actinomycetes, Kocuria sp., Conexibacter sp., Brevibacterium sp. and Rubrobacter sp.},
month = {July},
}
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