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@article{183681,
author = {R.Vijayalakshmi and Sri Ganga jothi.G},
title = {Molecular Identification and Phylogenetic Analysis of Bacopa monnieri Using DNA Barcoding},
journal = {International Journal of Innovative Research in Technology},
year = {2025},
volume = {12},
number = {3},
pages = {2768-2774},
issn = {2349-6002},
url = {https://ijirt.org/article?manuscript=183681},
abstract = {The majority of the population in the Tamil Nadu relies on herbal products as their primary source for their healthcare needs. After the recognition of sequence was submitted to the NCBI, and Gen Bank accession was obtained for the plant species Key words: Vitex negundo, Blast, DNA Bar coding, Leaves Bacopa monnieri L. as an important and effective alternative medicine for neuroprotection against dementia, amnesia, Parkinson's disease (PD), Alzheimer's disease (AD) and it prevent anxiety and stress. The efficiency of these leaf herbal powder is greatly reliant on the use of authentic plant material, and to this day no standard protocol has been established to authenticate plant materials. DNA barcoding offers a quick and reliable species authentication tool, but its application to plant material has been less successful due to lack of a standard DNA barcoding loci in plants and poor DNA yield from powdered plant products. In this project work, we use match gene-specific primers for Bacopa monnieri. the specified sequence is amplified using PCR and Agarose gel electrophoresis to get separated bands of sequence. then the amplified sequence was selected for NCBI – Basic Local Alignment Tool (BLAST). After trimming, the sequence was submitted to the NCBI, and Gen Bank accession was obtained for the plant species},
keywords = {Bacopa monnieri, Blast, DNA Bar coding, Leaves},
month = {August},
}
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