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@article{184447,
        author = {Rupal Jatvada and Dr Chetana Roat},
        title = {Comparative Assessment of Extraction Methods and Quantitative Estimation of Luteolin in the Leaves of Mentha piperita by HPLC},
        journal = {International Journal of Innovative Research in Technology},
        year = {2025},
        volume = {12},
        number = {4},
        pages = {1489-1494},
        issn = {2349-6002},
        url = {https://ijirt.org/article?manuscript=184447},
        abstract = {The study aims to identify the most effective organic solvent and extraction technique for isolating luteolin from the leaves of Mentha piperita through quantitative estimation using high-performance liquid chromatography (HPLC). The leaves were cleaned, dried, and powdered, followed by extraction using maceration, reflux, and Soxhlet techniques with four solvents of varying polarity: methanol, ethanol, chloroform, and ethyl acetate. The extract yield and luteolin content were analyzed through HPLC, which employed a Lichrospher C18 column and a methanol-acetic acid mobile phase.
The results indicate that the extraction method and solvent significantly influence luteolin yield and purity. The reflux technique using methanol yielded the highest purity (98.97%) and luteolin content (5.57% w/w). In comparison, the maceration and Soxhlet techniques produced lower yields and purity. Methanol emerged as the most efficient solvent for luteolin extraction, while other solvents such as ethanol, chloroform, and ethyl acetate yielded significantly lower luteolin content. Among the three methods, the reflux technique proved to be the most efficient in isolating luteolin.
In conclusion, the reflux method using methanol is recommended for the efficient extraction of luteolin from Mentha piperita leaves, making it suitable for applications in research and industry. These findings can aid in developing optimized extraction protocols for luteolin and other bioactive compounds in medicinal plants.},
        keywords = {Mentha piperita, luteolin, high-performance liquid chromatography, extraction techniques, reflux method, methanol, bioactive compounds.},
        month = {September},
        }