THE ROLE OF QUALITY DNA IN MOLECULAR PLANT TAXONOMY AND PHYLOGENETIC STUDIES

  • Unique Paper ID: 186484
  • PageNo: 1881-1884
  • Abstract:
  • Traditional approach in plant taxonomy for characterization, identification, classification and nomenclature of plants is based on phenotypic similarities and differences of plants. Phenotypic traits of plants may vary due to environmental conditions and often create major problems in proper identification. Recent advances in molecular techniques can help plant taxonomist to overcome these problems. Most of the molecular techniques are PCR based and one of the major challenges in molecular taxonomy and phylogenetic study is to extract quality DNA from voucher herbarium specimens, which are stored plant material for taxonomic studies. We have compared the one-tube extraction method with the traditional Cetyltrimethylammonium bromide (CTAB) method on different herbarium samples and found that the one-tube extraction method gave a good amount of DNA suitable for PCR as compared to CTAB method.

Copyright & License

Copyright © 2026 Authors retain the copyright of this article. This article is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

BibTeX

@article{186484,
        author = {Muley Mala and Rafiuddin Naser},
        title = {THE ROLE OF QUALITY DNA IN MOLECULAR PLANT TAXONOMY AND PHYLOGENETIC STUDIES},
        journal = {International Journal of Innovative Research in Technology},
        year = {2025},
        volume = {12},
        number = {6},
        pages = {1881-1884},
        issn = {2349-6002},
        url = {https://ijirt.org/article?manuscript=186484},
        abstract = {Traditional approach in plant taxonomy for characterization, identification, classification and nomenclature of plants is based on phenotypic similarities and differences of plants.
Phenotypic traits of plants may vary due to environmental conditions and often create major problems in proper identification. Recent advances in molecular techniques can help plant taxonomist to overcome these problems. Most of the molecular techniques are PCR based and one of the major challenges in molecular taxonomy and phylogenetic study is to extract quality DNA from voucher herbarium specimens, which are stored plant material for taxonomic studies.
We have compared the one-tube extraction method with the traditional Cetyltrimethylammonium bromide (CTAB) method on different herbarium samples and found that the one-tube extraction method gave a good amount of DNA suitable for PCR as compared to CTAB method.},
        keywords = {Molecular Taxonomy, DNA extraction, one tube method, PCR},
        month = {November},
        }

Cite This Article

Mala, M., & Naser, R. (2025). THE ROLE OF QUALITY DNA IN MOLECULAR PLANT TAXONOMY AND PHYLOGENETIC STUDIES. International Journal of Innovative Research in Technology (IJIRT), 12(6), 1881–1884.

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