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@article{192160,
        author = {M V V Satyaveni},
        title = {Innovative In Vitro Callus Induction and Molecular Characterization Frameworks for Advancing Plant Conservation and Genetic Understanding},
        journal = {International Journal of Innovative Research in Technology},
        year = {2026},
        volume = {12},
        number = {9},
        pages = {594-601},
        issn = {2349-6002},
        url = {https://ijirt.org/article?manuscript=192160},
        abstract = {Plant tissue culture is a cornerstone of modern plant biotechnology, playing a critical role in plant conservation, regeneration, and genetic improvement. Among in vitro techniques, callus induction represents a key developmental phase that enables cellular dedifferentiation, regeneration, and molecular-level investigations. However, conventional callus induction protocols frequently suffer from genotype dependency, oxidative stress, and genetic instability, limiting their reliability—particularly in conservation-oriented applications. This concept-driven article proposes an integrated experimental framework that combines innovative in vitro callus induction strategies with molecular characterization approaches to enhance culture efficiency while ensuring genetic fidelity. The framework emphasizes optimized explant selection and preconditioning, advanced plant growth regulator (PGR) combinations, media enrichment, and controlled culture systems, coupled with molecular marker analysis and gene expression profiling. Examples from endangered, medicinal, and model plant species are highlighted to demonstrate the broad applicability of the proposed approach. The integration of tissue culture innovation with molecular validation is presented as a robust and scalable strategy for advancing plant conservation and deepening genetic understanding.},
        keywords = {Callus induction; in vitro culture; molecular markers; genetic fidelity; plant conservation},
        month = {February},
        }