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@article{194381,
        author = {Dr Maya B Nair and Dr Sangeetha P M},
        title = {Isolation and Characterization of Soil Microorganisms with Potential Antimicrobial Activity},
        journal = {International Journal of Innovative Research in Technology},
        year = {2026},
        volume = {12},
        number = {10},
        pages = {4258-4267},
        issn = {2349-6002},
        url = {https://ijirt.org/article?manuscript=194381},
        abstract = {Soil is a very rich ecosystem and a famous source of microorganisms that produce antimicrobials. Due to the growing antimicrobial resistance rate, the search of the soil-based microbes as the source of new bioactive compounds has become interesting again. The objective of this study was to isolate, screen and characterize soil microorganisms that had antimicrobial activity against some pathogenic bacteria. The serial dilution and culture-based methods were used to process soil samples. Results were 28 microbial isolates that were screened using the cross-streak technique in the determination of antimicrobial activity. The agar well diffusion assay was also used to test potent isolates. Morphological, biochemical and molecular characterization, which included 16S rRNA gene sequencing, were done. The initial screening indicated that 32.1% of the isolates were antimicrobial active with bigger inhibition levels in Gram-positive bacteria. The production of extracellular antimicrobial metabolites was confirmed by secondary screening, with some exhibiting a broad-spectrum activity. Bacillus subtilis, Bacillus licheniformis and Streptomyces spp. were identified as the potent isolates by molecular identification. The microorganisms that obtain their location on soil are still promising sources of antimicrobial agents, and additional research is justified to define the bioactive compounds.},
        keywords = {Soil microorganisms; Antimicrobial activity; Actinomycetes; Bacillus; Streptomyces; Antibiotic resistance},
        month = {March},
        }