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@article{203345,
        author = {Mrs. Rupali Lavate and Hake Rutuja and Irkar kavita and Jadhav Tanisha and Jare Snehali and Dr. Amit Panaskar and Dr. Bhagyashri Panaskar},
        title = {In vitro nephroprotective activity and potential antioxidant activity of Martynia annua fruit extract},
        journal = {International Journal of Innovative Research in Technology},
        year = {2026},
        volume = {12},
        number = {12},
        pages = {11760-11766},
        issn = {2349-6002},
        url = {https://ijirt.org/article?manuscript=203345},
        abstract = {Background: Kidney disorders caused by drug toxicity, oxidative stress, diabetes, and environmental pollutants are increasing worldwide. Synthetic nephroprotective drugs are often associated with adverse effects and high cost. Therefore, there is a growing need for safe and effective plant-based nephroprotective agents. Martynia annua Linn., belonging to the family Martyniaceae, is traditionally used for various medicinal purposes and is known to possess antioxidant and therapeutic properties.
Objective: The present study aimed to evaluate the in vitro nephroprotective and antioxidant activity of ethanolic extract of Martynia annua fruit.
Materials and Methods: The fruits of Martynia annua were collected, shade dried, powdered, and extracted using 95% ethanol by Soxhlet extraction. Preliminary phytochemical screening was carried out to identify the presence of bioactive constituents. Antioxidant activity was evaluated using DPPH radical scavenging assay with ascorbic acid as standard. Nephroprotective activity was assessed against cisplatin-induced toxicity using HEK-293 cell lines by MTT assay.
Results: Phytochemical screening confirmed the presence of alkaloids, flavonoids, tannins, carbohydrates, saponins, and phenolic compounds. In the DPPH assay, the extract showed concentration-dependent antioxidant activity with maximum inhibition of 62.69% at 100 µg/mL and IC50 value of 74.04 µg/mL. In the MTT assay, the extract exhibited significant nephroprotective activity by increasing cell viability against cisplatin-induced toxicity. Maximum cell viability of 82.76% was observed at 100 µg/mL.
Conclusion: The findings of the study indicate that the ethanolic extract of Martynia annua fruit possesses significant antioxidant and nephroprotective activity, which may be attributed to the presence of flavonoids and phenolic compounds. The plant can be considered a potential natural source for the development of nephroprotective agents, although further pharmacological and clinical studies are required.},
        keywords = {Martynia annua, nephroprotective activity, antioxidant activity, DPPH assay, HEK-293 cell line, MTT assay, cisplatin.},
        month = {May},
        }