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@article{206452,
        author = {V S Bhadrashette and D U Gawai},
        title = {Extraction and Estimation of Total Phenolic Compounds from Different Plant Parts of Hemidesmus indicus (L.) R.Br.},
        journal = {International Journal of Innovative Research in Technology},
        year = {2026},
        volume = {13},
        number = {2},
        pages = {1925-1933},
        issn = {2349-6002},
        url = {https://ijirt.org/article?manuscript=206452},
        abstract = {Hemidesmus indicus (L.) R.Br., commonly known as Indian sarsaparilla or Anantamul, is a valuable medicinal plant widely used in Ayurvedic, Siddha, and folk medicine for the treatment of skin disorders, inflammation, urinary ailments, blood purification, fever, and general debility. The therapeutic significance of the plant is attributed to the presence of diverse phytochemicals, particularly phenolic compounds, flavonoids, tannins, glycosides, saponins, triterpenoids, and volatile constituents. Phenolic compounds are of special interest because of their antioxidant, antimicrobial, anti-inflammatory, and free-radical scavenging properties, which contribute significantly to the medicinal potential of plant extracts. The present study was undertaken to extract and estimate total phenolic compounds from different plant parts of Hemidesmus indicus (L.) R.Br., namely leaves, bark, fruits, roots, and stem, and to compare their extraction yield and phenolic richness.
Fresh plant materials were collected, authenticated, shade dried, powdered, and subjected to solvent extraction using a polar organic solvent under controlled conditions. The crude extracts obtained from each plant part were concentrated and weighed to determine extraction yield. Total phenolic content was estimated by the Folin–Ciocalteu colorimetric method, using gallic acid as standard, and the results were expressed as mg gallic acid equivalent per gram dry weight (mg GAE/g DW) Considerable variation was observed in extraction yield as well as total phenolic content among the plant parts studied. Leaves exhibited the highest extraction yield (26.0%) and maximum phenolic content (95.40 mg GAE/g DW), followed by bark (23.5% yield; 84.75 mg GAE/g DW), fruits (21.2% yield; 72.60 mg GAE/g DW), roots (19.5% yield; 60.85 mg GAE/g DW), and stem (17.2% yield; 52.40 mg GAE/g DW). A positive relationship was observed between extraction yield and phenolic content, indicating that phenolic compounds contributed substantially to the extractable phytochemical fraction of H. indicus.
The findings of the study suggest that phenolic compounds are differentially distributed among the plant parts of H.indicus, with leaves and bark serving as the richest sources. The results highlight the potential of these plant parts for use in antioxidant studies, phytochemical standardization, herbal formulations, and pharmaceutical applications. Furthermore, the use of aerial plant parts such as leaves may provide a more sustainable alternative to destructive root harvesting. The study contributes to the phytochemical characterization of H. indicus and provides a basis for further work on isolation, characterization, and biological evaluation of its phenolic constituents.},
        keywords = {H. indicus, total phenolic content, Folin–Ciocalteu reagent, gallic acid equivalent, medicinal plant, extraction yield, phytochemicals, antioxidant potential},
        month = {July},
        }