Copyright © 2026 Authors retain the copyright of this article. This article is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

@article{207981,
        author = {SANJAI T and D NIRAIMATHI and N.SAKTHIVEL and V.SANJAY},
        title = {Evaluation of the protective anti-haemolytic effect of Gymnema sylvestre against ouret lanata (L) kuntze-induced erythrocyte membrane damage},
        journal = {International Journal of Innovative Research in Technology},
        year = {2026},
        volume = {13},
        number = {3},
        pages = {3595-3602},
        issn = {2349-6002},
        url = {https://ijirt.org/article?manuscript=207981},
        abstract = {Erythrocyte membrane stability is widely used as an in-vitro index of the membrane-protective and anti-inflammatory potential of plant extracts, since the erythrocyte membrane shares several structural similarities with the lysosomal membrane. The present study evaluated the protective anti-haemolytic effect of Gymnema sylvestre against Ouret lanata (L.) Kuntze-induced erythrocyte membrane damage. Objective: To prepare ethanolic extracts of G. sylvestre leaves and O. lanata flowers, screen them phytochemically, and evaluate the haemolytic potential of O. lanata and the membrane-protective potential of G. sylvestre on human erythrocytes in vitro. Methods: Extracts were obtained by Soxhlet extraction and subjected to preliminary phytochemical screening. O. lanata (25–400 µg/mL) was evaluated for haemolytic activity on washed human erythrocytes; 100 µg/mL O. lanata, corresponding to 46.0% haemolysis, was selected to establish a standardized erythrocyte injury model. Low, medium and high concentrations of G. sylvestre were then co-incubated with the injury model, and haemoglobin release was measured spectrophotometrically at 540 nm. Results: O. lanata produced concentration-dependent haemolysis, rising from 13.0% at 25 µg/mL to 84.0% at 400 µg/mL. G. sylvestre extract alone showed concentration-dependent membrane stabilization (30–82% protection across 25–400 µg/mL), approaching the 85% protection shown by the reference standard. Against O. lanata-induced injury, G. sylvestre reduced haemolysis from 46.0% to 35.0%, 23.0% and 11.0% at low, medium and high concentrations respectively, corresponding to 23.9%, 50.0% and 76.1% membrane protection. Conclusion: Gymnema sylvestre exhibits concentration-dependent erythrocyte membrane-stabilizing activity against Ouret lanata (L.) Kuntze-induced membrane damage, supporting its potential as a membrane-protective and anti-inflammatory agent warranting further pharmacological investigation.},
        keywords = {Gymnema sylvestre; Ouret lanata (L.) Kuntze; erythrocyte membrane stabilization; anti-haemolytic activity; membrane protection; Soxhlet extraction.},
        month = {August},
        }