Antimicrobial activity, Fenugreek, Extraction, Phytochemicals, Inhibition zone
Fenugreek (Trigonella foenum-graecum L.) is one of the most promising medicinal herbs known from ancient times having nutritional value. Fenugreek seeds are known to exhibit potent antioxidant, antimicrobial, hypoglycemic, and nephroprotective activities. This study aimed at determining the physicochemical properties of fenugreek seeds in order to ascertain its quality as a food and medicinal agent. The purpose of this study was to screen phytochemical compounds present in crude fenugreek seeds extracts and evaluate their potential activity against an E. coli, Bacillus cereus, and Staphylococcus aureus.Organic (hexane, acetone, ethanol and methanol) extracts were prepared from powdered fenugreek seeds. Bacterial growth inhibitory effects were evaluated by measuring the diameter of the inhibition zone (IZ) using an agar-disc diffusion method. The organic extracts prepared with chloroform, acetone or methanol showed low to moderately high growth inhibitory effect. Phytochemical screening of the extracts revealed the presence of the major compounds known to have anti-bacterial activity such as tannins and flavonoids in the aqueous and methanolic extracts. The results indicate that fenugreek seeds crude extracts may have anti-bacterial potential against selective micro organism.