Exiguobacterium aquaticum CS6 a potent calcite solubilizer isolated calcite isolated from marble slurry
Harshada Joshi
Exiguobacterium aquaticum CS6, Calcite Solubilizing bacteria, Marble slurry, Nutrient agar
Marble industries are growing in pace with the increasing demand of marble stones. During the mining process the slurry is discharged as a waste either to a vacant land or to a surface water source in the nearby areas. The current investigation was aimed to detect calcite solubilizing bacteria from marble slurry collected from dumping yard in Sukher, Udaipur, India. Isolation was performed on nutrient agar medium using pour plate method. Calcite solubilizing efficiency (CSE) and index (CSAI) of the isolate was tested on 0.1% calcite agar medium and incubation was done at 37°C for 10 days. The isolate was identified on the basis of morphological, biochemical and molecular methods. Isolate CS6 was appeared on nutrient agar medium after an incubation of 24 hours at 37°C. The colony appeared as orange, pinpointed and with convex elevation. It showed clear zone of 32 mm diameter after 10 days of incubation indicating calcite solubilizing activity. The maximum CSE and CSAI observed were 166% and 260 respectively. Isolate CS6 was identified as Exiguobacterium aquaticum CS6 (accession no. MK353511). The isolate showed remarkable calcite solubilizing activity and can be further analyzed for its possible use in restoration of marble slurry contaminated soil.
Article Details
Unique Paper ID: 153965

Publication Volume & Issue: Volume 8, Issue 9

Page(s): 361 - 363
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