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A Simple UV Spectrophotometric Method Development and Validation of Melatonin in its Bulk and Pharmaceutical Dosage Forms

  • Unique Paper ID: 161156
  • Volume: 10
  • Issue: 2
  • PageNo: 775-778
  • Abstract:
  • A simple and sensitive UV Spectrophotometric method has been developed for the quantitative estimation of Melatonin in its bulk and pharmaceutical dosage form. The drug showed maximum absorbance at 225nm. Regression analysis of Beer’s plot showed correlation of 0.998 in the concentration range of 1.5-7.5 μg/ml and the percentage recovery was between 98-102% indicting high degree of accuracy. The % RSD of intra-day and inter-day precision was found to be less than 2 as per ICH guidelines. Sensitivity of the method was confirmed by accurate LOD and LOQ values which were obtained 0.23 and 0.698 respectively.
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Cite This Article

  • ISSN: 2349-6002
  • Volume: 10
  • Issue: 2
  • PageNo: 775-778

A Simple UV Spectrophotometric Method Development and Validation of Melatonin in its Bulk and Pharmaceutical Dosage Forms

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Impact Factor
8.01 (Year 2024)
UGC Approved
Journal no 47859

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