EFFECT OF DIFFERENT FERMENTATION STRATEGIES ON GROWTH OF BACILLUS THURINGENSIS ISRAELENSIS AND ITS TOXICITY TOWARDS AEDES AEGYPTI
Author(s):
GOPINATHAN C, ANAGHA ANIRUDHAN, HANA MOL K E
Keywords:
Bacillus thurengiensis israelensis, batch fermentation, fed batch fermentation, solid state fermentation, cell growth.
Abstract
The harmful effects of chemical pesticides on the environment and life had led to the search for biopesticides. Bacillus thurengiensis israelensis (Bti) is a biopesticide used to control mosquitoes worldwide. The main advantage of Bti over chemical insecticide is its specific activity towards diphteran insects. The growing importance of these biopesticide in insect control activities has encouraged in aiming to develop new methods to produce it in low cost. In this study we aim to to study the effect of batch, fed-batch, submerged and solid state fermentation on the growth of Bti using rotten grape juice as substrate. Significantly, higher biomass was obtained in fed batch compared with other fermentation techniques. Glucose medium showed less activity compared with others. Fed batch cultivation with intermittent feeding at 12 hrs of cultivation recorded highest cell density of 1.16 at 600 nm. These results support Bti growth using rotten grape juice as substrate can be used for cost effective production of Bti against mosquito larvae.
Article Details
Unique Paper ID: 161251

Publication Volume & Issue: Volume 10, Issue 3

Page(s): 328 - 336
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