M.Pandiyan, A.Karthikeyan, C.Sivakumar, A.Krishnaveni, M.Sivaji, P.Sivakumar, A.Bharathi, K.Natarajan, M.Mahalingam, Indira gandhi, A.Velayutham
Pre fertilization barrier –Vigna radiata – Vigna umbellata- Vigna vexillata- Pollen failed on stigma – Poor stigma receptivity
In this study investigating the possibility of developing interspecific fertile hybrids between Vigna radiata with other wild Vigna species to produce durable resistance against Mungbean Yellow Mosaic Virus and bruchids resistant genotypes in the greengram. Among the wild Vigna species, Vigna. umbellata having durable MYMV resistance and bruchids resistance in Vigna vexillata To eradicate MYMV problem in Vigna radiata , the Vigna umbellata involved to make a more number of crosses with greengram and Vigna vexillata. Recovery of fertile F1s is highly challengeable in Vigna radiata with other wild Vigna species crosses. Crosses among wild species with cultivated are unable to easily generate fertile hybrids. Even though no crossability barriers problem occur in a matting time. But both level of F1s of fertile and sterile hybrids were produced in Vigna radiata x Vigna umbellata and Vigna radiata x Vigna vexillata crosses with more number of accession used. The crossability percentage may varied depends on close relation of wild species with greengram. To identify the reproductive barriers blocking the pod development and seed set in in F1s, pollen viability and pistil acceptability and receptivity of same flower was studied in F1s. Pollen-pistil interactions in direct and reciprocal crosses of Vigna radiate, Vigna umbellata and Vigna vexillata were investigated to characterize pollen-stigma compatibility. Premature surface portions of stigma cells and pollen were observed to reveal cytological mechanism regulating pistil receptivity and the pistil and pollen interactions. We observed that stigma receptivity, pollen viability and varied depending on the species. Various reciprocal crosses showed different pollen-stigma in compatibilities expressed their pollen germination rates compared to direct crosses. Some of the cross pollen grains germinated on female stigmas, the pollen tubes were arrested in the pistils and pollen tubes not able to reach the ovaries which could not carried out backcross further . So the embryo sacs remained unfertilized until degenerating. The F1s were affected by pre-fertilization barriers. Pollen pistil incompatibility always towards low pollen fertility and poor stigma recepti
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Unique Paper ID: 162119

Publication Volume & Issue: Volume 10, Issue 6

Page(s): 545 - 552
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