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Method Development And Validation of Antidiabetic Drug in its Pharmaceutical Dosage Form By Suitable Analytical Method

  • Unique Paper ID: 165953
  • Volume: 11
  • Issue: 1
  • PageNo: 2148-2152
  • Abstract:
  • The paper describes the invention of a simple, precise, and sensitive approach for detecting saxagliptin in bulk medication and marketed formulations using reverse-phase liquid chromatography. The separation was done on Epic C12 (250 mm×4.6 mm×5 µm) using mobile phase (Methanol: buffer) in the ratio of 80:20 (v/v). The run time was 7 minutes, and the wavelength for saxagliptin was taken as 230 nm. A literature review finds that there are very few HPLC techniques available using this composition of mobile phase. As a result, an attempt was undertaken to create an RP-HPLC technique for saxagliptin. The devised method was validated in terms of accuracy, precision, linearity, system suitability, LOD and LOQ, robustness, and assay. The linearity ranged from 1-3 μg/ml, with a correlation value of 0.999. The designed and validated RP-HPLC method is used to identify the eluted.
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Cite This Article

  • ISSN: 2349-6002
  • Volume: 11
  • Issue: 1
  • PageNo: 2148-2152

Method Development And Validation of Antidiabetic Drug in its Pharmaceutical Dosage Form By Suitable Analytical Method

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12 Jun, 2024

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Impact Factor
8.01 (Year 2024)
UGC Approved
Journal no 47859

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