Development of validated Spectrophotometric Methods: Simultaneous Equation Method and Q-Absorption Ratio method and for Simultaneous Estimation of Secnidazole and Ciprofloxacin.
Author(s):
Megha K Patel, Kiran J Suthar, Vishal T Chudasama, Bharat Rajpurohit, Ashok Patel
Keywords:
Ciprofloxacin, Q-Absorption Ratio method, Secnidazole, Simultaneous, Simultaneous Equation method, Validation.
Abstract
Two simple, accurate, sensitive and economical spectrophotometric methods have been developed and subsequently validated for determination of Ciprofloxacin and Secnidazole in bulk. For the simultaneous equation method, the estimation of Secnidazole and Ciprofloxacin was carried out at 319 nm (λ max of Secnidazole) and 271.2 nm (λ max of Ciprofloxacin). For Q - absorption ratio method, estimation of Secnidazole and Ciprofloxacin at 282 was carried out at 319 nm (λ max of Secnidazole) and 282 nm (isosbestic point of both drugs). Calibration curves of Secnidazole and Ciprofloxacin were found to be linear in the concentration ranges of 1-25 𝜇g/mL, with their correlation coefficient values (r2) being more than 0.995. In the precision study, the % RSD value was found within limits (RSD < 2%). The percentage recovery for Secnidazole at various concentration levels varied from 100.14 to 102.14 % and 1.1.2 to 102.13 by Method I and II respectively, While The percentage recovery for Ciprofloxacin varied from 100.52 to 101.51 and 100.3 to 101.33 by Method I and II respectively confirming that the projected methods are termed as an accurate and it can be applied successfully for the simultaneous estimation of Secnidazole and Ciprofloxacin in pure combined form.
Article Details
Unique Paper ID: 166970

Publication Volume & Issue: Volume 4, Issue 5

Page(s): 282 - 290
Article Preview & Download


Share This Article

Join our RMS



Conference Alert

NCSEM 2024

National Conference on Sustainable Engineering and Management - 2024

Last Date: 15th March 2024

Latest Publication

Call For Paper

Volume 11 Issue 1

Last Date for paper submitting for Latest Issue is 25 June 2024

About Us

IJIRT.org enables door in research by providing high quality research articles in open access market.

Send us any query related to your research on editor@ijirt.org

Social Media

Google Verified Reviews

Contact Details

Telephone:6351679790
Email: editor@ijirt.org
Website: ijirt.org

Policies

Important Links

Browse Rsearch papers