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Method Development and Validation for The Simultaneous Estimation of Milbemycin Oxime and Lufenuron in Bulk and Tablet Dosage Forms By RP-HPLC

  • Unique Paper ID: 168924
  • Volume: 11
  • Issue: 5
  • PageNo: 2176-2180
  • Abstract:
  • In the present study a simple, accurate and precise reverse phase high performance liquid chromatographic method has been developed and validated for the simultaneous estimation of Milbemycin oxime and Lufenuron in bulk and tablet dosage form. The method was developed using Waters HPLC system on a Inertsil – C18, ODS column (150 x 4.6 mm, 5µ) using Methanol and Water in the ratio of 70:30 v/v as mobile phase in isocratic elution mode at a flow rate of 1.0ml/min with a load of 20µl. The detection was carried out at 353nm. The retention time of Milbemycin Oxime and Lufenuron were found to be 3.005 min and 5.291 min respectively. The developed method was validated with respect to linearity, robustness, precision and accuracy and was successfully applied for the simultaneous quantitative determination of Milbemycin Oxime and Lufenuron in the tablet dosage form.
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Cite This Article

  • ISSN: 2349-6002
  • Volume: 11
  • Issue: 5
  • PageNo: 2176-2180

Method Development and Validation for The Simultaneous Estimation of Milbemycin Oxime and Lufenuron in Bulk and Tablet Dosage Forms By RP-HPLC

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