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A BLOTTING TECHNIQUES TO DETECTIONS AND IDENTIFICATION OF BIOMOLECULES

  • Unique Paper ID: 181652
  • Volume: 12
  • Issue: 1
  • PageNo: 4810-4819
  • Abstract:
  • Biological macromolecules constitute large molecules essential for life that are constructed from smaller organic molecules. The four primary categories of biological macromolecules include carbohydrates, lipids, proteins, and nucleic acids (DNA and RNA). Carbohydrates derive from monosaccharides, lipids from fatty acids, proteins from amino acids, and nucleic acids from nucleotides.In molecular biology laboratories, scientists often need to isolate specific molecules from particular macromolecules such as protein, DNA, or RNA. This process is termed "Blotting," a methodology whereby macromolecules like DNA, RNA, or proteins are separated in a gel matrix, subsequently transferred to a solid support, and detected using a specific probe. Blotting techniques enable researchers to identify and characterize specific molecules within complex mixtures through a three-step procedure: initial separation via gel electrophoresis, transfer of the gel onto a membrane/paper, and visualization of the target molecule using a specific visualizing agent. The nomenclature of blotting varies according to the target molecule: protein analysis is designated "Western Blotting" or "Immunoblotting," DNA analysis is termed "Southern Blotting," and RNA analysis is referred to as "Northern Blotting.”
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Cite This Article

  • ISSN: 2349-6002
  • Volume: 12
  • Issue: 1
  • PageNo: 4810-4819

A BLOTTING TECHNIQUES TO DETECTIONS AND IDENTIFICATION OF BIOMOLECULES

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Impact Factor
8.01 (Year 2024)
UGC Approved
Journal no 47859

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