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@article{173629,
        author = {Uday kumar B V and Shashank S and Chethankumar M and Darshan G P},
        title = {CRISPR-Cas Systems in Modulating Fruit Ripening: Insights from Banana Research},
        journal = {International Journal of Innovative Research in Technology},
        year = {2025},
        volume = {11},
        number = {10},
        pages = {926-934},
        issn = {2349-6002},
        url = {https://ijirt.org/article?manuscript=173629},
        abstract = {Bananas (Musa spp.) are vital to global food security and trade, but their climacteric ripening leads to rapid spoilage and post-harvest losses. Conventional ripening control methods, such as cold storage and chemical treatments, often lack efficiency and sustainability. CRISPR/Cas9-based genome editing has emerged as a precise and promising alternative for modulating fruit ripening by targeting key ethylene biosynthesis genes.
This review examines the molecular regulation of banana ripening, emphasizing the role of MaACO1 and MaACS1 in ethylene production. CRISPR-mediated knockouts of these genes have demonstrated significant potential in delaying ripening without introducing foreign DNA, offering a more efficient and publicly acceptable alternative to conventional breeding and transgenic methods.We further discuss the challenges and future perspectives of CRISPR applications in banana improvement, including regulatory frameworks, off-target effects, and consumer acceptance. Integrating genome editing with existing post-harvest strategies can reduce losses, enhance sustainability, and improve the economic viability of banana production. This review provides insights into the latest advancements in CRISPR-Cas systems for fruit ripening modulation, with a focus on their transformative potential for the banana industry.},
        keywords = {CRISPR/Cas9, banana ripening, ethylene biosynthesis, post-harvest losses, genome editing.},
        month = {March},
        }