HPTLC Methods To Assay Active Ingredients In Pharmaceutical Formulations: A Review Of The Method Development And Validation Steps

  • Unique Paper ID: 173874
  • PageNo: 1803-1820
  • Abstract:
  • High-performance thin-layer chromatography (HPTLC) is still increasingly finding its way into pharmaceutical analysis in some parts of the world. With the advancements in the stationary phases and the introduction of densitometers as detection equipment, the technique achieves for given applications a precision and trueness comparable to high-performance liquid chromatography (HPLC). In this review, the literature is surveyed for developed and validated HPTLC methods to assay active ingredients in pharmaceutical formulations published in the period 2005–2011. Procedures and approaches for method development, validation, and quantitative assays are compared with the standard ways of conducting them. Applications of HPTLC in some other areas are also briefly highlighted.

Copyright & License

Copyright © 2026 Authors retain the copyright of this article. This article is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

BibTeX

@article{173874,
        author = {Yogesh Chaudhari and Tina Gajanan Shete and Dinesh Gajanan Masram and Vrushabh Sanghai and Srushti Shrikant Patil and Sonawane Sumit Bapurao and Pratik Deepak Dhokne and Swapnil S. Tirmanwar},
        title = {HPTLC Methods To Assay Active Ingredients In Pharmaceutical Formulations: A Review Of The Method Development And Validation Steps},
        journal = {International Journal of Innovative Research in Technology},
        year = {2025},
        volume = {11},
        number = {10},
        pages = {1803-1820},
        issn = {2349-6002},
        url = {https://ijirt.org/article?manuscript=173874},
        abstract = {High-performance thin-layer chromatography (HPTLC) is still increasingly finding its way into pharmaceutical analysis in some parts of the world. With the advancements in the stationary phases and the introduction of densitometers as detection equipment, the technique achieves for given applications a precision and trueness comparable to high-performance liquid chromatography (HPLC). In this review, the literature is surveyed for developed and validated HPTLC methods to assay active ingredients in pharmaceutical formulations published in the period 2005–2011. Procedures and approaches for method development, validation, and quantitative assays are compared with the standard ways of conducting them. Applications of HPTLC in some other areas are also briefly highlighted.},
        keywords = {},
        month = {March},
        }

Cite This Article

Chaudhari, Y., & Shete, T. G., & Masram, D. G., & Sanghai, V., & Patil, S. S., & Bapurao, S. S., & Dhokne, P. D., & Tirmanwar, S. S. (2025). HPTLC Methods To Assay Active Ingredients In Pharmaceutical Formulations: A Review Of The Method Development And Validation Steps. International Journal of Innovative Research in Technology (IJIRT), 11(10), 1803–1820.

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