Phytochemical Constituents and Antibacterial Activity of Aqueous and Ethanolic Extracts of Moringa oleifera Leaves

  • Unique Paper ID: 180050
  • PageNo: 178-182
  • Abstract:
  • The present study aimed to evaluate the antibacterial activity of Moringa oleifera (family: Moringaceae) leaf extracts and to perform phytochemical screening. Moringa oleifera is a widely utilized plant in tropical regions, valued in Ayurvedic medicine for its therapeutic properties. Its leaves are known for their nutritional benefits and potential antibiotic effects. In this study, aqueous and ethanolic extracts of M. oleifera leaves were prepared and tested against a panel of bacterial strains: Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Proteus vulgaris, Streptococcus mutans, Bacillus subtilis, and Staphylococcus epidermidis. Phytochemical analysis of both extracts revealed the presence of bioactive compounds including flavonoids, tannins, steroids, alkaloids, and saponins. The antibacterial activity was assessed using the agar well diffusion method. Both extracts exhibited inhibitory effects against all tested bacterial strains. Notably, the ethanolic extract demonstrated maximum antibacterial activity against Streptococcus mutans, whereas the aqueous extract showed the highest activity against Proteus vulgaris. These findings suggest that M. oleifera leaves possess broad-spectrum antibacterial potential, likely due to the presence of multiple phytoconstituents. Further studies focusing on compound isolation and mechanism of action are warranted to explore its potential in antimicrobial drug development.

Copyright & License

Copyright © 2026 Authors retain the copyright of this article. This article is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

BibTeX

@article{180050,
        author = {Ravi Hashappa and Srikanth P},
        title = {Phytochemical Constituents and Antibacterial Activity of Aqueous and Ethanolic Extracts of Moringa oleifera Leaves},
        journal = {International Journal of Innovative Research in Technology},
        year = {2025},
        volume = {12},
        number = {1},
        pages = {178-182},
        issn = {2349-6002},
        url = {https://ijirt.org/article?manuscript=180050},
        abstract = {The present study aimed to evaluate the antibacterial activity of Moringa oleifera (family: Moringaceae) leaf extracts and to perform phytochemical screening. Moringa oleifera is a widely utilized plant in tropical regions, valued in Ayurvedic medicine for its therapeutic properties. Its leaves are known for their nutritional benefits and potential antibiotic effects. In this study, aqueous and ethanolic extracts of M. oleifera leaves were prepared and tested against a panel of bacterial strains: Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Proteus vulgaris, Streptococcus mutans, Bacillus subtilis, and Staphylococcus epidermidis. Phytochemical analysis of both extracts revealed the presence of bioactive compounds including flavonoids, tannins, steroids, alkaloids, and saponins. The antibacterial activity was assessed using the agar well diffusion method. Both extracts exhibited inhibitory effects against all tested bacterial strains. Notably, the ethanolic extract demonstrated maximum antibacterial activity against Streptococcus mutans, whereas the aqueous extract showed the highest activity against Proteus vulgaris. These findings suggest that M. oleifera leaves possess broad-spectrum antibacterial potential, likely due to the presence of multiple phytoconstituents. Further studies focusing on compound isolation and mechanism of action are warranted to explore its potential in antimicrobial drug development.},
        keywords = {Antibacterial Activity, Moringa oleifera, Phytochemical Screening},
        month = {May},
        }

Cite This Article

Hashappa, R., & P, S. (2025). Phytochemical Constituents and Antibacterial Activity of Aqueous and Ethanolic Extracts of Moringa oleifera Leaves. International Journal of Innovative Research in Technology (IJIRT), 12(1), 178–182.

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