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@article{181587,
        author = {RudraPratap Singh Parihar and Aakanksha Mandal},
        title = {Investigate antihyperlipidemic activity of Crataeva Nurvala Bark by high fat high sugar diet induced model},
        journal = {International Journal of Innovative Research in Technology},
        year = {2025},
        volume = {12},
        number = {1},
        pages = {5315-5327},
        issn = {2349-6002},
        url = {https://ijirt.org/article?manuscript=181587},
        abstract = {In order to create a rat model that is appropriate for pharmacological screening, the current study set out to reproduce the natural history and metabolic features of type 2 diabetes in humans. The male Sprague-Dawley rats (160-180 g) were split into two groups and fed either the in-house made high-fat diet (HFD) (58% calories as fat) or the commercially available normal pellet diet (NPD) (12 % calories as fat) for two weeks. Insulin (PI), triglycerides (PTG), body weight, total cholesterol (PTC), and basal plasma glucose (PGL) were significantly increased in the HFD-fed rats when compared to the NPD-fed control rats. The HFD rats also shown a significant decrease in the intravenous insulin glucose tolerance test (IVIGTT) glucose disappearance rate (K-value). Hyperinsulinemia and a decreased glucose disappearance rate (K-value) indicated that rats fed a high-fat diet developed insulin resistance. A subgroup of the rats from both groups received an intraperitoneal injection of a low dose of streptozotocin (STZ) (35 mg kg(-1)) following two weeks of dietary manipulation. When STZ was injected into insulin-resistant HFD-fed rats, they experienced frank hyperglycemia; in contrast, NPD-fed rats experienced a moderate increase in PGL. After receiving a STZ injection, the PI level in HFD rats significantly decreased, but only to a level that was similar to that of control rats fed NPD. Furthermore, following STZ therapy in HFD-fed rats, the levels of PTG and PTC were further exacerbated. However, in rats given NPD, STZ (35 mg kg (-1), i.p.) did not significantly change the levels of PI, PTG, and PTC. These fat-fed/STZ-treated rats thereby mimic the metabolic traits and natural course of the disease that are typical of people who are more likely to acquire type 2 diabetes due to obesity and insulin resistance. Furthermore, it was discovered that both insulinotropic (glipizide) and insulin sensitizing (pioglitazone) medications had the ability to reduce glucose levels in the fat-fed/STZ-treated rats. These diabetic rats also demonstrated the impact of glipizide and pioglitazone on the plasma lipid parameters (PTG and PTC). The current study shows that a combination of a rat fed a high-fat diet and receiving a low dose of STZ can be used as an alternative animal model for type 2 diabetes that mimics the human condition and can be used to test anti-diabetic medications for sort 2 diabetes treatment.},
        keywords = {},
        month = {June},
        }