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@article{185594,
        author = {Ms. Arthita Dey and Mr. Debraj Modak},
        title = {MICROPROPAGATION OF NEEM (MATURE) BY SHOOT-TIP CULTURE (Azadirachta indica)},
        journal = {International Journal of Innovative Research in Technology},
        year = {2025},
        volume = {12},
        number = {5},
        pages = {2160-2164},
        issn = {2349-6002},
        url = {https://ijirt.org/article?manuscript=185594},
        abstract = {Azadirachta indica (Neem) is a valued medicinal tree of immense ecological and medicinal importance.[1] Because of the limitations of traditional propagation, this research focused on developing an effective micropropagation protocol with shoot tip explants by tissue culture methods.[2] Explants were taken from healthy stock plants(=20 years) and plated on Murashige and Skoog (MS) medium with different concentrations of BA (1.5 mg/L, 2.0 mg/L, 2.5 mg/L, and 3.0 mg/L) and a singular concentration of NAA (0.1 mg/L). Each of the concentrations was tested with shoot tip explants, surface sterilized and grown under controlled conditions (25 + 2 °C, 16/8-hour light/dark cycle) for 25 days. Among the treatments, MS medium containing 2.0 mg/L BA demonstrated the highest morphogenic response, with explants producing up to 4 branches and 17 leaves, reaching a final length of 8.6 cm from an initial length of 4.5 cm. This study highlights the effectiveness of 2.0 mg/L BA in promoting shoot proliferation and growth, establishing a reliable protocol for large-scale propagation and conservation of neem.},
        keywords = {Azadirachta indica, micropropagation, shoot tip culture, Murashige and Skoog (MS) medium, BA,NAA, in vitro propagation, plant tissue culture.},
        month = {October},
        }